fermentation help please!

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e*e
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fermentation help please!

Post by e*e » Tue Oct 24, 2006 11:18 am

we just made a fermentation experiment with glucose molarities of 0.2, 0.4, 0.6, 0.8 and 1 M. We saw that the gas formed is the most in 0.2 M and is decreasing as the molarity increases. What is the reason that as molarity increases, amount of gas formed decreases? Shouldnt the amount of gas formed in a certain interval of time which was about 10 mins in our experiments increase up to a certain point and then become constant since this is an enzyme-substrate relationship? Please help! Urgent!

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Post by SWORD MASTER » Tue Oct 24, 2006 10:17 pm

Me too.. i always find some diffuclty with fermentation :cry:
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druid
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Post by druid » Wed Oct 25, 2006 3:36 am

Well, it seems you just kill the poor yeasts by hypertonic solution...
:~(

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Post by e*e » Wed Oct 25, 2006 5:00 pm

That is probably true but can you kill a yeast with a 0.6 molar glucose solution??

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MrMistery
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Post by MrMistery » Wed Oct 25, 2006 5:36 pm

yeah, since the medium is more hypertonic than the cell, and the yeast cell undergoes plasmolysis.
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druid
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Post by druid » Wed Oct 25, 2006 6:40 pm

e*e wrote:That is probably true but can you kill a yeast with a 0.6 molar glucose solution??


Check what optimal glucose concentration has to be for yeast. As
I remember it is ~0.1 M ( not sure ).

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Post by e*e » Thu Oct 26, 2006 4:55 pm

ok thanky you very much. i ll check that :)

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