Antibodies can determine the activation state of an enzyme??

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medbio
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Antibodies can determine the activation state of an enzyme??

Post by medbio » Sun Oct 22, 2006 9:15 pm

Hello!

I have a statement which may be true or false. The statement is "antibodies can be used to determine the activation state of an enzyme". My opinion is that this is true. Well, antibodies can be used if appropriately raised against the active conformation of an enzyme. The primary antibody can be made to bind only to active site of enzyme and the secondary antibody will bind to the primary one with a reporter enzyme, for ex. peroxidase. If signal is detected it means that the enzyme is active and if not it is inactive.

But I am not sure for that.. Can anyone help me? :)
cArpE diEm

sdekivit
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Post by sdekivit » Mon Oct 23, 2006 3:15 pm

well you can determine the active state of enzymes. well examples are for example aintibodies raised against phosphorylated kinases. But you also have to bear in mind that these antibodies may also recognize the inactive form of the enzyme.

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Dr.Stein
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Post by Dr.Stein » Thu Oct 26, 2006 8:59 am

@sdekivit: Are you familiar with "ubiquitin sysem"? I need a help. Thank you in advance ;)
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