Primer selection question (homework)

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: Leonid, amiradm, BioTeam

Post Reply
kevin86
Garter
Garter
Posts: 1
Joined: Sun Oct 15, 2006 5:58 am

Primer selection question (homework)

Post by kevin86 » Sun Oct 15, 2006 6:00 am

If you can help me, then thanks

Image

oppox
Death Adder
Death Adder
Posts: 96
Joined: Sat May 13, 2006 11:20 am
Location: Sweden

Post by oppox » Mon Oct 16, 2006 9:00 am

They made it simplier by writing the start of the gene in bold letters.

The extra G in the end is for raising the annealing temp in the PCR and is not found in the gene.

The gene and vector is written 5'-3' as the primers.

A gene is inserted 5'-3' into a vector, that is as its written.

The forward primer is the most easy to find.

Good luck, if u have more specific questions, ask them.

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest