peroxisomal targeting sequence in the thiolase enzyme

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aboredprincess
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peroxisomal targeting sequence in the thiolase enzyme

Post by aboredprincess » Sat Oct 14, 2006 12:19 am

How do I identify the region of the thiolase protein that contains peroxisomal targeting sequence

druid
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Post by druid » Sat Oct 14, 2006 8:40 pm

By PTS2 which is Arg-Leu-(X)5-His-Leu.

http://www.pubmedcentral.nih.gov/articl ... tid=545895

aboredprincess
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Post by aboredprincess » Sun Oct 15, 2006 12:00 am

Let me be more specific. I have data however, I do not know how to interpret that data to determine the region of the thiolase protein that contains the peroxisomal targeting sequence.

druid
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Post by druid » Sun Oct 15, 2006 12:18 am

What kinda data do you have? Be more specific.

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Post by aboredprincess » Sun Oct 15, 2006 1:30 am

Thanks for your help: I wish I could draw this table, but I'll do my best to describe it to you.

There are ten horizontal bar graphs with part of the graph in white and the other part in gray. The white area is thiolase; the gray area is HDH.
1----200
The first bar l thiolasel HDH l -
50---200
2nd l thiolase l HDH l -
100--200
thiolase l HDH -
125--200
thiolase l HDH +
150--200
thiolase l HDH +
1---150
thiolasel HDH -
1--125
thiolasel HDH -
1---100
thiolas l HDH +
1--50
thiolassl HDH +
1--25
thiolasel HDH +
The numbers above each protein indicate the amino acids from thiolase present in the hybrid protein.
+ indicates growth on medium lacking histidine
- indicates lack of growth on medium lacking histidine.

druid
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Post by druid » Sun Oct 15, 2006 1:56 pm

What is HDH?
Suppose HDH must be present in cytoplasm for histidine synthesis, enabling the cell to live on his-lacking medium.
Suppose hybride protein still has HDH functionality.
Then cutting fragments from thiolase one can suddenly remove peroxysome targeting sequence (PTS), hybride protein will stay in cytoplasm and the cell will survive without histidine.
If this factual framework is correct then according to the data PTS is located at 1-25 and 150-200 residues. The sygnals are autonomous (each independently of other drives protein into peroxisome).

aboredprincess
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Post by aboredprincess » Sun Oct 15, 2006 3:21 pm

I don't fully understand. Why are PTS located at 1-25 and 150-200 and not 1-50 and 125-200 or the other proteins that indicate growth on medium lacking histidine?

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Post by druid » Sun Oct 15, 2006 4:05 pm

If PTS was on 1-50 then hybride protein would be transfered into peroxisome and the cell couldnt grow on Histidine lacking medium. But data says the cell does grow.
Thats first-order logic ;)

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Post by aboredprincess » Sun Oct 15, 2006 6:10 pm

thanks for the help.

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