Eadie-Hofstee plot

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tetedupet
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Eadie-Hofstee plot

Post by tetedupet » Fri Oct 13, 2006 2:40 pm

I have been told to calculate the Km of an enzme using a Eadie-Hofstee plot... however I'm not really sure. I know I need to work out the gradient of the slope, but that gives -Km... so do I just flip the signs or what?

Thanks

kiekyon
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Post by kiekyon » Fri Oct 13, 2006 3:33 pm

yes, u r right!! :lol:

sdekivit
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Re: Eadie-Hofstee plot

Post by sdekivit » Sun Oct 15, 2006 9:15 pm

tetedupet wrote:I have been told to calculate the Km of an enzme using a Eadie-Hofstee plot... however I'm not really sure. I know I need to work out the gradient of the slope, but that gives -Km... so do I just flip the signs or what?

Thanks


i posted a post about enzyme kinetics and the different plots inclusive the mathematical background in this section. try the search option.

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