clonning

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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kabuto
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clonning

Post by kabuto » Wed Sep 27, 2006 4:15 pm

1. does digested clonning vector need to be purified?
2. why only onion genome with fragment size 1-3kb used to build genomic library?

oppox
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Post by oppox » Wed Sep 27, 2006 5:37 pm

1. well I guess the enzymes could cut again if u dont, I dont remember how I did. Some enzymes can be stopped by for an example heating. you do best to download the manual to your enzymes

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canalon
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Post by canalon » Thu Sep 28, 2006 12:38 pm

1-It is better as said by oppox, but sometimes heat inactivation is used followed by ligation in order to avoid loss of material during purification
2- The longer the fragment, the harder to insert it into a vector in a stable manner.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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