SOS:hairpin PCR template

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dzrdez
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SOS:hairpin PCR template

Post by dzrdez » Fri Sep 22, 2006 7:58 am

:?
My experiment need a hairpin as a PCR template, but after PCR there is
no the target. while annealing, the template formed an intrastrand haipin and thus primers were unable to associate with it.who did ever do ?how did resolve it?
:( why? primer concentration low, or annealing temed improper???

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