DNA denautration by pH

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princess.lauren
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DNA denautration by pH

Post by princess.lauren » Mon Sep 18, 2006 4:17 pm

Hi!
during my bio lecture (2nd yr) the prof mentioned that denaturation of DNA can occur by increasing temp or increasing pH. I understand that at higher temps the Hbonds will break between nucleotides, but how does denaturation occur at higher pH? i guess im looking for the process.
thanks
lauren

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zami'87.
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Re: DNA denautration by pH

Post by zami'87. » Tue Sep 19, 2006 6:55 pm

Adenine becomes soluble in weak acid...and uracil(speaking of RNA) in ammonia
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Re: DNA denautration by pH

Post by kiekyon » Wed Sep 20, 2006 2:33 am

princess.lauren wrote:Hi!
during my bio lecture (2nd yr) the prof mentioned that denaturation of DNA can occur by increasing temp or increasing pH. I understand that at higher temps the Hbonds will break between nucleotides, but how does denaturation occur at higher pH? i guess im looking for the process.
thanks
lauren


heat, high pH, low pH, they all break the H bond

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