Optimising Constructs for soluble expression

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Docuk
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Optimising Constructs for soluble expression

Post by Docuk » Tue Sep 05, 2006 12:34 pm

What bioinformatic techniques do people use to optimise their constructs for soluble protein expression.

I generally do predictive secondry structure mapping, domain mapping and finally run the protein sequence through a predictive disorder programme?

Are there more successful techniques?

Thanks

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