Two problems im trying to figure out.

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oppox
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Two problems im trying to figure out.

Post by oppox » Mon Sep 04, 2006 8:22 am

Hello Im having a little problem. I trying to express a protein from a gete in a pET28 vector. Im growing it in Bl21DE3 and inducing it with IPTG. I get no protein when I analyze it on a SDS-gel, any suggestions?

Also, Im having some problems preparing the samples for the SDS gel, that is when Im starting from pellets. I only get kind of a sticky goo which I cant apply on the gel, I have to dilute it so the bands get kind of weak.
How do u prepare a sample from bacterial pellets?

Thanks in advance

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