CYP2D6 amplification

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ahitz
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CYP2D6 amplification

Post by ahitz » Thu Aug 24, 2006 10:14 am

Hi,

I am trying to amplify CYP2D6 (about 5kb) using Roche's Expand High fidelity PCR system. So far no success. See different bands but not the one I want. Also used different PCR programs. Tried different MgCl2 conc. Nothing. So what I am looking for now is a PCR protocol for this nasty gene! Would be great if even used same Taq.. primers I used are the ones used by Lundqvist et al. (1999). PLEASE HELP!!!!!

weesper
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Post by weesper » Thu Aug 24, 2006 9:04 pm

Tried a touch-down PCR?

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