## how to prepare...

Debate and discussion of any biological questions not pertaining to a particular topic.

kabuto
Coral
Posts: 118
Joined: Sun Apr 02, 2006 7:30 am

### how to prepare...

how to prepare

a)100ml of 2M glucose solution(glucose molecular weight=180)
b)20ml 10% SDS

is it
for a),
get 36g of glucose, dissolve in water, top it up to 100ml

b)
no idea???

pls help..

LilKim
Coral
Posts: 436
Joined: Mon Mar 20, 2006 8:36 pm
Contact:
A is correct

B. If you are starting with a 100% (or any SDS solution that is greater than 10%) SDS solution. You can use the (c1)(v1)=(c2)(v2) to figure this out.

c1= 100% (the concentration of the initial SDS solution)
v1= x
c2= 10% (the desired final concentration)
v2= 20ml (the desired total volume)

Solve for v1 (x)... and when you get that value, dilute it in water to TOTAL volume of 20ml.

You can also think of a 10 percent solution as 1 part solute + 9 parts solvent. Therefore: if you needed to make a 100ml 10% SDS solution you would add 1 part SDS for every 9parts of water. So you'd end up adding 10parts SDS and 90parts water (ie 10grams/mLs SDS + 90grams/mLs H2O)

hope this helps!

- kim

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