*** Length of DNA Fragments ***

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Dustfinger
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*** Length of DNA Fragments ***

Post by Dustfinger » Sat Aug 12, 2006 10:47 pm

Hi people, got a problem (again): :(

A piece of DNA (8000 bases) is cut with the restriction enzyme BglI and then with HpaII and finally with the mixture of both. The fragments are separated from each other , then a Southern Blot is done (again with these enzymes and with the mixture of them).
You can see the results in the two images I´ve attached to my post. (Image 1: restriction with BglI and HpaII results; image2: Southern Blot result)
My task is now to find out the length of the DNA fragments in the Southern Blot with the mixture of the enzymes. (The fragments thus are not shown on the image; there´s a blank column where they belong.)

Could anybody help me ? Thanks !

Here are the images :
uacp.php?u=7174
Image
Image

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canalon
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Post by canalon » Sat Aug 12, 2006 11:45 pm

the images were not attached properly. It does not open.
Patrick

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any proof. (Ashley Montague)

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Post by Dustfinger » Sun Aug 13, 2006 6:31 am

oh damn...
here they are again: ( the wrong way roun, but starts at No 1 logically )
Attachments
image2.gif
Southern Blot
image2.gif (2.28 KiB) Viewed 2232 times
image3.gif
image3.gif (2.27 KiB) Viewed 2232 times
image1.gif
restriction with BglI and hpaII
image1.gif (2.88 KiB) Viewed 2234 times
Image
Image

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canalon
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Post by canalon » Sun Aug 13, 2006 12:57 pm

Fig 1 gives you the fragments n the lane 3, and the possibility to draw a restrictio map.
Fig lane 1 and 2 allows you to see which fragments are recognized by your probe in the southern blot. Now you just have to look at your restriction map and locate the annealing site of your probe and locating them on the double digest.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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Post by Dustfinger » Mon Aug 14, 2006 10:51 am

Hi, can anybody solve this example I got (-> images 1, 2 and 3) and tell the length of these fragments ? Cause with me it´s always the same: I need an example with solution and then I can do the other ones similarly.
Image
Image

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Post by Dustfinger » Mon Aug 14, 2006 8:43 pm

Are they 2.7 and 0.3 kb long ? Am I right in this conclusion ?
Image
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Post by Dustfinger » Tue Aug 15, 2006 8:42 am

Hey people ! It´s over now, got the answer myself. Any Mod may now delete this thread.
Image
Image

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