Gateway cloning

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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DocSmith
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Gateway cloning

Post by DocSmith » Sat Aug 12, 2006 7:40 pm

I am going to start a new task and I am planning to use Gateway cloning method, which I have not tried before. I like to know some tricks and tips on Gateway cloning method.

Thanks a lot!

Irene123
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Post by Irene123 » Sat Aug 12, 2006 11:24 pm

Check out this site: http://bioinfoman.com/bm/bm_cg6.php

What can BioInfoMan help you in your Gateway Cloning tasks?
Design PCR primers with terminal attB sites
Clone PCR fragments into an Entry Clone via the BP Reaction.
Perform LR recombination reaction with selected Entry Clone and Destination Vector.
Perform BP recombination reaction with selected Expression Clone and Donor Vector.
Automatically generate cloning constructs: either Entry Clone or Expression Clone.

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