DNA/RNA

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Diana Timpano
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DNA/RNA

Post by Diana Timpano » Sun Apr 24, 2005 3:56 pm

Hi....A couple of questions..help....

If a structural gene contains 300 DNA nucleotides, how many amino acids will be used in the protein synthesis process?

If a protein has 150 amino acids, how many DNA nucleotides would make up the structural gene?
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Arty
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Re: DNA/RNA

Post by Arty » Sun Apr 24, 2005 4:59 pm

Diana Timpano wrote:Hi....A couple of questions..help....

If a structural gene contains 300 DNA nucleotides, how many amino acids will be used in the protein synthesis process?

If a protein has 150 amino acids, how many DNA nucleotides would make up the structural gene?


Pretty easy!!
No of amino acids= 300/3 = 100 amino acids
No of nucleotides= 150*3 = 450 nucleotides
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zami'87.
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Re: DNA/RNA

Post by zami'87. » Sun Apr 24, 2005 7:51 pm

Arty wrote:
Diana Timpano wrote:Hi....A couple of questions..help....

If a structural gene contains 300 DNA nucleotides, how many amino acids will be used in the protein synthesis process?

If a protein has 150 amino acids, how many DNA nucleotides would make up the structural gene?


Pretty easy!!
No of amino acids= 300/3 = 100 amino acids
No of nucleotides= 150*3 = 450 nucleotides

if we don't go in details(stop codon...)?
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biostudent84
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Post by biostudent84 » Sun Apr 24, 2005 9:02 pm

448 maximum if you include the start codon and stop codons.
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canalon
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Post by canalon » Sun Apr 24, 2005 9:09 pm

biostudent84 wrote:448 maximum if you include the start codon and stop codons.


I think if you really want to count stop codons (start codons are transcribed) you get these results
(300 nt - 1 non transcribed stop codon)= 99 aa
and (150aa + 1 non transcribed stop codon)x3= 453nt

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biostudent84
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Post by biostudent84 » Mon Apr 25, 2005 2:47 am

What are the "n" and the "t" meaning in this example?
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canalon
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Post by canalon » Mon Apr 25, 2005 3:25 am

biostudent84 wrote:What are the "n" and the "t" meaning in this example?


oopps "nt" stands for nucleotides (bases) as "aa" stands for amino acids. :oops:

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biostudent84
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Post by biostudent84 » Mon Apr 25, 2005 1:32 pm

I'd like to know where I got that number from and what the heck I was thinking..... :( :( :( :( :(

98 maximum if you count the start and stop codons.

Sorry.....
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canalon
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Post by canalon » Mon Apr 25, 2005 2:17 pm

biostudent84 wrote:I'd like to know where I got that number from and what the heck I was thinking..... :( :( :( :( :(

98 maximum if you count the start and stop codons.

Sorry.....


:!: Start codons ARE transcribed, hence 99.
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biostudent84
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Post by biostudent84 » Mon Apr 25, 2005 5:16 pm

They are? Thanks
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MrMistery
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Post by MrMistery » Wed Apr 27, 2005 8:12 pm

From what i know, start codons are transcripted, but if your protein does not begin with metionin then it is later removed
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cytochromeP
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INTRONS

Post by cytochromeP » Thu May 05, 2005 3:33 pm

r we forgetting something...
Oh yes...INTRONS
if the structural gene contains introns then the no. of aa reqd. for protein making is indeterminate !
or else if its a bacterial gene then my predecessors have already done the math.
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