Restriction Enzymes

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Ajay
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Restriction Enzymes

Post by Ajay » Fri Aug 04, 2006 12:20 am

Can anyone furnish details about "Recognition Sites in Restriction Enzymes" ?

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canalon
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Post by canalon » Fri Aug 04, 2006 1:37 am

The name seems self explanatory, no? Restriction enzyme recognize a specific sequence in the DNA and cut it. This recognition can be affected by methylation of the DNA molecule. If you want a list of restriction sites, you can use thi site: http://rebase.neb.com/rebase/rebase.html
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Navin
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Post by Navin » Fri Aug 04, 2006 6:51 am

Something special about restriction sites: They are palindromic sequences.
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Post by Ajay » Fri Aug 04, 2006 2:44 pm

i would like to be more clear in my question. I mean the recognition sites IN restriction enzymes which recognizes the specific dna sequence for eg. palindromic sequences

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Post by Navin » Fri Aug 04, 2006 2:47 pm

So you are refering to the active site of the restriction enzymes?
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Post by Ajay » Sat Aug 05, 2006 12:58 am

Yes exactly... to be more precise "the region of binding in restriction enzymes and the region of cleaving"

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