Transfection of cells while in cell-cycle arrest?

Discussion of all aspects of cellular structure, physiology and communication.

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noamd
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Transfection of cells while in cell-cycle arrest?

Post by noamd » Tue Aug 01, 2006 3:15 pm

Hi,
I am interested in transfecting a human cell line (U20s or MCF7) that is arrested at G1,S,and G2.
have you ever done this successfully?
how did you arrest the cells?
how did you transfect?
what was your efficiency compared to normally growing cells.
I am grateful for your time, this is crucial for my project,
Noam Diamant.
8)

weesper
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Post by weesper » Tue Aug 15, 2006 10:15 am

Both these cell lines are great for electroporation; electroporation really doesnt need cells to be in G1/S but you can synchronize them after; you can look this up anywhere. Take care mxj

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