Immunoprecipitation

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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littlefish
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Immunoprecipitation

Post by littlefish » Mon Jul 31, 2006 3:20 pm

I got problems with my coIP experiment. There were always a lot of background bands.
Any ideal of how to get rid of background bands in coIP?
Thanks.

kk
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Joined: Fri Sep 23, 2005 7:58 pm

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Post by kk » Tue Aug 08, 2006 2:11 am

I had the same problem. You have to make sure, that there is protease inhibitor present to avoid degradation.

My question would be:

at what size does the IgG run on the western (my immunoprecipitating antibody is mouse IgG2A)??

I hope not around 60 kDa....

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