degraded fish dna

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alittlepenguin
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degraded fish dna

Post by alittlepenguin » Wed Jul 26, 2006 6:36 pm

hi,

i've been running pcr's on fish dna for a couple of weeks now. recently, i've found that i'm either getting very little product or smears following the same protocol. i've heard that fish dna degrades quite quickly and i'm wondering if anyone else has had similar experiences and found any solutions.

i have a stock of dna which was extracted about 2 months ago sitting in my freezer. i've been using that stock diluted 1:50

thanks

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