proteinase k

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proteinase k

Post by AnaFrancisca » Thu Jul 20, 2006 3:00 pm


We're optimising a DNA extraction protocol, using proteinase k.

It would be important for us to know if a 14000 rpm centrifugation inactives proteinase k.

We couldn't find anything about this property of proteinase K, maybe because it is not usually important :roll: , but we thank any information or bibiliography that mentions this issue.

Thank you!!!!


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Inland Taipan
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Post by canalon » Thu Jul 20, 2006 5:28 pm

No enzymes are not sensitive to centrifugation. But the enzyme will not go in the pellet either

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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