proteinase k

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
AnaFrancisca
Garter
Garter
Posts: 2
Joined: Fri Apr 28, 2006 5:04 pm

proteinase k

Post by AnaFrancisca » Thu Jul 20, 2006 3:00 pm

Hi,

We're optimising a DNA extraction protocol, using proteinase k.

It would be important for us to know if a 14000 rpm centrifugation inactives proteinase k.

We couldn't find anything about this property of proteinase K, maybe because it is not usually important :roll: , but we thank any information or bibiliography that mentions this issue.

Thank you!!!!

:)

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Thu Jul 20, 2006 5:28 pm

No enzymes are not sensitive to centrifugation. But the enzyme will not go in the pellet either
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Post Reply

Who is online

Users browsing this forum: No registered users and 9 guests