Why does NaOH loosen the cell wall?

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galileosn
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Why does NaOH loosen the cell wall?

Post by galileosn » Sun Jul 02, 2006 7:48 pm

Why does NaOH loosen the cell wall and releases the plasmid DNA? What happens at the molecular level?

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MrMistery
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Post by MrMistery » Wed Jul 05, 2006 7:56 pm

Do you mean in bacteria ori in yeasts?
in bacteria i think it distories the peptide cross-links between the N-acetylglucosamine+N-acetyl muramic polimers. But i am not sure.
In yeasts no idea...
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kell
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Post by kell » Fri Aug 11, 2006 7:49 am

I need some help MrMistery. Im on an assignment on cell wall. can u gimme a list of sites that is good for me to get enough info bout it?
if u can get me an article bout cell wall evolution,it's much more appreciated...
:-) :D

sdekivit
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Post by sdekivit » Fri Aug 11, 2006 9:47 am

MrMistery wrote:Do you mean in bacteria ori in yeasts?
in bacteria i think it distories the peptide cross-links between the N-acetylglucosamine+N-acetyl muramic polimers. But i am not sure.
In yeasts no idea...


true :) the esterbondings are destroyed resulting in unstability of the cell wall. Due to osmosis, lysis will occur

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MrMistery
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Post by MrMistery » Sat Aug 12, 2006 7:19 pm

@kell
Just type "cell wall" in google. You will get 80.000.000 results. Those should get you started ;)
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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