About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.
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- Joined: Tue Aug 09, 2005 5:01 am
Hi. Does anyone know the use of affinity tags and affinity chromatography for isolating beta-lactamases? Have anyone come across such papers? Hope I can be able to have some information about them. Thanks.
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- Joined: Mon Jul 03, 2006 2:48 am
- Location: Philippines
Gee.. sorry I couldn't help. I was working with enzyme detection (Taq polymerase) too in the past and I didn't reach that step, all my cultures died. Maybe you ought to screen for beta-lactamase production first; but I guess your past that step. I would recommend searching in EpNet if you have account access; they have almost all the journals available online.
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- Joined: Fri Jul 07, 2006 3:23 am
- Location: IL, USA
There is an old paper that may be of help:
Biochem J. 1984 Jul 15;221(2):505-12
It's suppose to work well with large amount of crude cell extracts.
I can e-mail the pdf file if you don't fine it.
Science is a wonderful thing if one does not have to earn one's living at it. (A. Einstein)
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