Isolation of bacterial enzymes???

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Isolation of bacterial enzymes???

Post by eTiT » Sun Jul 02, 2006 1:46 pm

Hi. Does anyone know the use of affinity tags and affinity chromatography for isolating beta-lactamases? Have anyone come across such papers? Hope I can be able to have some information about them. Thanks.

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Post by mkwaje » Mon Jul 03, 2006 6:33 am

Gee.. sorry I couldn't help. I was working with enzyme detection (Taq polymerase) too in the past and I didn't reach that step, all my cultures died. Maybe you ought to screen for beta-lactamase production first; but I guess your past that step. I would recommend searching in EpNet if you have account access; they have almost all the journals available online.

good luck.

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Post by Doctor G » Fri Jul 07, 2006 3:31 am

There is an old paper that may be of help:

Biochem J. 1984 Jul 15;221(2):505-12

It's suppose to work well with large amount of crude cell extracts.

I can e-mail the pdf file if you don't fine it.
Science is a wonderful thing if one does not have to earn one's living at it. (A. Einstein)

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