protein after fractionation

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protein after fractionation

Post by statesman » Sat Jun 24, 2006 3:36 am

Imagine protein X, destined to go to the plasma membrane of a cell. Assume that the mRNA carrying the genetic message for protein X has already been translated by ribosomes in a cell culture. You collect the cells, break them open, and then fractionate their contents by differential centrifugation. In the pellet of which fraction would you expect to find protein X? Explain your answer by describing the transit of protein X through the cell.

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Post by Mjhavok » Sat Jun 24, 2006 3:46 am

Can I ask what you do or study?
Forgive him, for he believes that the customs of his tribe are the laws of nature!

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Post by majik1213 » Fri Jun 30, 2006 1:00 pm

You can expect the following results:
Pellet 1: nuclei, whole cells
Pellet 2: most of the mitochondria, some peroxisomes and lysosomes
Pellet 3: some of the mitochondria, most peroxisomes and lysosomes
Pellet 4: rough ER, smooth ER, and Golgi Apparatus
Final Supernatent: soluble proteins

So, the protein won't be in any of the pellets, but rather you will find it in the final supernatent.

Source: ... erFrac.GIF

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