apoptosis

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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salix59
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apoptosis

Post by salix59 » Tue Jun 13, 2006 11:21 am

hello every body.I am working on apoptosis on a cell line .and I encounter problemes in detecting DNa fragmentation if you have any recomendation that you have test it before plz help me
Thank you up front.

Monday
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Post by Monday » Fri Jun 30, 2006 1:38 am

you mean you can't see the ladder?Maybe you extrat the cellular DNA at a wrong time.

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mkwaje
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Post by mkwaje » Mon Jul 03, 2006 3:55 am

If I'm not mistaken, apoptosis does not generally mean destruction or fragmentation of the DNA, but cell lysis. Its possible though that DNAse and RNAse are released during lysis; this will degrade your DNA but I assumed you added a buffer that would inactivate this and preserv your DNA. One way that I can recommend to check for apoptosis would be ocular inspection to see for lyzed cells. Hope that helps answer your question :)

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canalon
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Post by canalon » Tue Jul 04, 2006 7:43 pm

mkwaje wrote:If I'm not mistaken, apoptosis does not generally mean destruction or fragmentation of the DNA, but cell lysis. [...]


You are mistaken. See our dictionnary about it
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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mkwaje
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Post by mkwaje » Wed Jul 05, 2006 1:34 pm

yep.. my mistake. thanks for the correction.

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