RT-PCR Primers concentration?

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HELISA
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RT-PCR Primers concentration?

Post by HELISA » Mon Jun 12, 2006 10:16 pm

Hi Guys,
Tomorrow I am planning to do my first RT-PCR reaction.
I have everything I need, except one important information, which I am still trying to obtain from internet.
Maybe someone knows, what should be the concentration of my primers stock which I should use for RT-PCR?
What is the final concentration of primers in the reaction?
Thank you a lot for any suggestions.
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oppox
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Post by oppox » Mon Jun 12, 2006 11:45 pm

Cant you find it on http://www.novagen.com or http://www.invitrogen.com in their product catalog, Ive never done TR-PCR so I dont know but these catalogs are sometimes very good. Goodl uck

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