## Doubling time for cells

**Moderators:** honeev, Leonid, amiradm, BioTeam

### Doubling time for cells

Can anyone help me out as how to find the doubling time of a particular cell line?

Thanks.

Thanks.

- MrMistery
- Inland Taipan
**Posts:**6832**Joined:**Thu Mar 03, 2005 10:18 pm**Location:**Romania(small and unimportant country)-
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What species are you working with? Is it some bacteria? Because some bacterial species have been studied and the average generation time published.

"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

2. split. and put a certain amount in each flask.(call it B)

3. After a few days, count the cells again.(call it C)(Record also the number of days. call it D)

B x 2to the power N = C

Solve for N. N is the number of doubles.

So it takes D days to have N number of doubles)

If it takes you 3 days to have 5 doubles, 1 double will take you 3/5 days. Which is 0.6day

0.6 day will be the doubling time.

**exponential growing phase**.

It could be hard to get a reliable calculus with adherent cells compare to

**suspension cells**.

Split your cells, measure their concentration : c1

Let them grow during h hours

Count them again : c2

**The doubling time(in hours)=h*ln(2)/ln(c2/c1)**

You can use decimal or neperien logarithm.

You can use this formula to forcast the cell expension or to calculate the volume to split to get a target concentration after a period of growing.

A software do this for you at http://www.doubling-time.com

For a given cell lines, the doubling time changes regarding the number of passages, the serum lot, the way you pass them...

For cell line info, check on : http://www.dsmz.de/human_and_animal_cell_lines/cell_line_index.php

### Re: Doubling time for cells

You can use the online calculator to calculate the doubling time of your cells.

http://www.doubling-time.com/compute.php?lang=en

here is the chinese version !

http://www.doubling-time.com/compute.php?lang=zh

Very convenient if you have several time points.

http://www.doubling-time.com/compute.php?lang=en

here is the chinese version !

http://www.doubling-time.com/compute.php?lang=zh

Very convenient if you have several time points.

### Re: Doubling time for cells

Hello, does anyone here can explain to me what is the information can we get from the cell doubling time t..is it telling us a measurement/or indicator ?...say: t measure a successful of radiation treatment? .i am not in biological area, i am a mathematics student. So can anyone here could explain to me please.....

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