Doubling time for cells

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kpatel
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Doubling time for cells

Post by kpatel » Tue Jun 06, 2006 3:37 pm

Can anyone help me out as how to find the doubling time of a particular cell line?

Thanks.

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LilKim
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Post by LilKim » Tue Jun 06, 2006 4:15 pm

I don't know where you can "look-up" that type of info.

But, it's pretty easyy to figure it out.

You'll just have to have a way of counting the cells... and then re-counting them every hour for like... 8-10 hours. And you should be able to get an approximate time.

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MrMistery
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Post by MrMistery » Tue Jun 06, 2006 5:12 pm

What species are you working with? Is it some bacteria? Because some bacterial species have been studied and the average generation time published.
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kpatel
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Post by kpatel » Wed Jun 07, 2006 3:10 pm

It is a rat Schwann cell line I am working with.

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MrMistery
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Post by MrMistery » Wed Jun 07, 2006 5:08 pm

You could try finding it in the literature, but i doubt you would have much luck. But you could determine it experimentally...
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Post by Priscilla » Fri Jul 28, 2006 6:32 pm

1. count the cells when it's confluent.(call it A)
2. split. and put a certain amount in each flask.(call it B)
3. After a few days, count the cells again.(call it C)(Record also the number of days. call it D)
B x 2to the power N = C
Solve for N. N is the number of doubles.
So it takes D days to have N number of doubles)
If it takes you 3 days to have 5 doubles, 1 double will take you 3/5 days. Which is 0.6day
0.6 day will be the doubling time.

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Post by snoopline » Fri Nov 10, 2006 1:25 am

Doubling time computing is correct only in exponential growing phase.
It could be hard to get a reliable calculus with adherent cells compare to suspension cells.
Split your cells, measure their concentration : c1
Let them grow during h hours
Count them again : c2

The doubling time(in hours)=h*ln(2)/ln(c2/c1)

You can use decimal or neperien logarithm.
You can use this formula to forcast the cell expension or to calculate the volume to split to get a target concentration after a period of growing.

A software do this for you at http://www.doubling-time.com

For a given cell lines, the doubling time changes regarding the number of passages, the serum lot, the way you pass them...
For cell line info, check on : http://www.dsmz.de/human_and_animal_cell_lines/cell_line_index.php

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Re: Doubling time for cells

Post by snoopline » Thu Dec 04, 2008 2:57 pm

You can use the online calculator to calculate the doubling time of your cells.
http://www.doubling-time.com/compute.php?lang=en
here is the chinese version !
http://www.doubling-time.com/compute.php?lang=zh

Very convenient if you have several time points.
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Adah
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Re: Doubling time for cells

Post by Adah » Mon Jul 05, 2010 10:31 am

Hello, does anyone here can explain to me what is the information can we get from the cell doubling time t..is it telling us a measurement/or indicator ?...say: t measure a successful of radiation treatment? .i am not in biological area, i am a mathematics student. So can anyone here could explain to me please.....

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JackBean
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Post by JackBean » Mon Jul 05, 2010 10:45 am

well, it can tell you, how preferable is that environment ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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