Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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I am looking for an E. coli strain for efficient transformation (recA) AND good expression of gene under lac promotor that can also deliver good quality plasmids. Any hints?
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Usually, you can kill off all bacteria that are NOT part of your experiment. You might have one or two left, but let them divide a few times, and you can quickly come up with a good culture.
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