E. coli strain for plasmid prep AND expression

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
vernet
Garter
Garter
Posts: 1
Joined: Wed Dec 08, 2004 4:38 pm

E. coli strain for plasmid prep AND expression

Post by vernet » Wed Dec 08, 2004 4:46 pm

I am looking for an E. coli strain for efficient transformation (recA) AND good expression of gene under lac promotor that can also deliver good quality plasmids. Any hints?

User avatar
biostudent84
Site Admin
Site Admin
Posts: 974
Joined: Thu Nov 11, 2004 6:00 am
Location: Farmville, VA
Contact:

Post by biostudent84 » Wed Dec 08, 2004 5:47 pm

Usually, you can kill off all bacteria that are NOT part of your experiment. You might have one or two left, but let them divide a few times, and you can quickly come up with a good culture.

Post Reply

Who is online

Users browsing this forum: No registered users and 5 guests