TRIzol

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HELISA
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TRIzol

Post by HELISA » Wed May 24, 2006 7:25 pm

Has anyone used TRIzol Reagen for total RNA extraction from plant material?
What makes this reagent so special, that it allows to avoid RNA degradation?
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LilKim
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Post by LilKim » Fri May 26, 2006 2:08 am

i've used it a hundred millions of time for total nucleic acid extraction of mammalian cells. (and i know of people who have used it for plants)

After extraction i would DNAse treat the sample to eat the DNA and keep RNA

but that's all I know...

- kim

HELISA
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Post by HELISA » Fri May 26, 2006 5:45 pm

Hmm...Still, I'm waiting for some good explanation, why TRIzol is worth buying.
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canalon
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Post by canalon » Fri May 26, 2006 8:19 pm

I went at Sigma, and saerched for trizol. And I found 3 references, including 2 in a free acces journal.
So May I suggest you to do your own research rather than waiting for people to do it for you?
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

HELISA
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Post by HELISA » Sun May 28, 2006 5:01 pm

Nice to meet you too...
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canalon
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Post by canalon » Sun May 28, 2006 8:07 pm

HELISA wrote:Hmm...Still, I'm waiting for some good explanation, why TRIzol is worth buying.


As if that was nice. Asking a question is OK, complaining becasue you still do not have an answer is not. We are not at your service.
I was trying to give you some leads to find an answer to your question since I do not know the answer. Now I don't have time to do all your work.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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LilKim
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Post by LilKim » Sun May 28, 2006 10:02 pm

Canalon ... you're great! (... but i'm sure you already knew that...!)

tranjo
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Post by tranjo » Sat Jun 03, 2006 4:05 pm

Trizol is worth buying because it's a standard for RNA work.

Best, J

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Post by 2810712 » Sat Jun 03, 2006 5:26 pm

How does trizol look like? I'm curious but its far away from my prescribed syllabus presently...still interested...
It extracts DNA & RNA...from both animal & plant...hehe i'm too litle here!
Is sigma some kind of Database?

Ved

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canalon
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Post by canalon » Sun Jun 04, 2006 3:23 am

trizol is a liquid, actually a solvent used in nucleic acids extractions.
And Sigma is one of the biggest manufacturer of fine chemicals for (life) sciences. So their catalogue is a very good start to find info on many chemicals.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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Post by tranjo » Sun Jun 04, 2006 4:44 am

Yes Sigma has a nice feature on the upper right column of each product page. The links include PDFs for uses, inserts, and MSDS. It's a good place to learn about the various chemicals that could end your life!

Best, J

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