antioxidants

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
ress
Garter
Garter
Posts: 9
Joined: Wed May 10, 2006 7:35 am

antioxidants

Post by ress » Wed May 10, 2006 7:37 am

why do we add antioxidants in the bufers for DNA extraction. what effect do they have on the contaminants

User avatar
victor
King Cobra
King Cobra
Posts: 2668
Joined: Sat Apr 30, 2005 12:01 pm
Location: Yogyakarta, Indonesia..
Contact:

Post by victor » Wed May 10, 2006 2:52 pm

usually antioxidants are used to react with high energy free radical atoms/molecules. The resuld is, those antioxidants become a free radical molecules but with lower reactivity compared to the previous free radicals.
Q: Why are chemists great for solving problems?
A: They have all the solutions.

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Wed May 10, 2006 3:56 pm

Hi and Tahnks are nice, when posting a question.

And what weird protocol are using to extract DNA because I do extract DNA relatively often, and I don't use any of those...
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

ress
Garter
Garter
Posts: 9
Joined: Wed May 10, 2006 7:35 am

Post by ress » Thu May 11, 2006 5:53 am

Canalon wrote:Hi and Tahnks are nice, when posting a question.

And what weird protocol are using to extract DNA because I do extract DNA relatively often, and I don't use any of those...


Sir,
I am extracting DNA from a highly phenolic plant. Protocols for such plants suggest use of antioxidants to keep phenolics in check. But, I wonder do these chemicals only affect phenolics or they also are playing role other than this.

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Thu May 11, 2006 11:44 am

I never worked with pants (phenolic or not), but to extract animal cells or bacterial DNA you do not need to use anti oxydants. I imagine that some of those phenolic compounds can get oxidized during procedure and attack your DNA, hence the antioxydants that are protecting your material.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

scottyiu
Coral
Coral
Posts: 103
Joined: Thu Dec 01, 2005 1:11 pm
Location: HK

Post by scottyiu » Sat May 13, 2006 8:08 am

What are free radicals?
This forum is so cool

User avatar
Poison
Inland Taipan
Inland Taipan
Posts: 2322
Joined: Sun Jan 02, 2005 12:44 pm
Location: Turkey

Post by Poison » Sat May 13, 2006 5:40 pm

It matters not how strait the gate
How charged with punishment the scroll
I am the Master of my fate
I am the Captain of my soul.

Post Reply

Who is online

Users browsing this forum: No registered users and 4 guests