protein extraction without addding proteinase inhibitor

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zoeyqqq
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protein extraction without addding proteinase inhibitor

Post by zoeyqqq » Thu May 04, 2006 10:22 pm

I did a protein extraction using RIPA buffer. I realize that I forgot to add proteinase inhibitor into the buffer.
I know that may cause a problem. But has anyone here ever try this before?
What should I do? Trash it or go ahead.
Thanks

Code of Life
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Hi

Post by Code of Life » Tue May 09, 2006 4:44 pm

I'd start it all over again mate...............also set up the new experiment with existing sample just as to be sure how much protein is lost by not adding protease inhibitor........its just a suggestion though.....wait for more replies......good luck

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