EGFR degradation

Discussion of all aspects of cellular structure, physiology and communication.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 3
Joined: Tue May 02, 2006 3:58 pm

EGFR degradation

Post by sem.meln » Tue May 02, 2006 4:03 pm

I transfect the cells with EGFR (there isn’t endogenous) and, by giving EGF, try to observe the EGFR internalization (by immunofluorescence IF) and subsequent degradation (by IF and Western), (was widely described in literature). To my surprise, I don’t see degradation, but increase in EGFR amount. Whether someone encounter a similar thing or have any idea?
Thank you,

King Cobra
King Cobra
Posts: 586
Joined: Sat Jul 30, 2005 7:16 pm
Location: holland

Post by sdekivit » Wed May 03, 2006 8:22 pm

hmmm normally EGFR is internalized and degraded by the ubiquitin mechanism

Post Reply

Who is online

Users browsing this forum: No registered users and 5 guests