Guanidine-HCl solution storage

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Joined: Fri Apr 28, 2006 5:04 pm

Guanidine-HCl solution storage

Post by AnaFrancisca » Fri Apr 28, 2006 5:13 pm

Hi, everybody: :D
I´ve been trying to extract DNA from solid tissue using a 8M solution of guanidine hydrochloride with ammonium acetate or alternatively with sodium acetate, pH 8.
The aim of using this solution is to purify the DNA, removing proteins.
It works most of the times, except when the solution is stored for several days at 4ºC. In this case, no DNA is obtained. :roll:
I wonder how this solution should be stored for this not to happen or if it is better to use a fresh one.
Thank you!

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Joined: Sat Apr 29, 2006 2:57 am

Post by helleborine » Thu May 04, 2006 2:14 am

Are you sure that's the reason? My lab mates and myself share a solution of Guanidine-HCl and we keep in on the shelf for months...

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