how to culture bacteria from sewage

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purna
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how to culture bacteria from sewage

Post by purna » Mon Apr 24, 2006 10:16 am

i would like to know how i can grow bacteria from sewage water, if i get a pellet from the sample water

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LilKim
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Post by LilKim » Mon Apr 24, 2006 6:19 pm

could you grow it in LB?

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canalon
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Post by canalon » Mon Apr 24, 2006 9:42 pm

you should probably resuspend in saline or whatever good solution that would not cause too much of an osmotic shock, and then plate on selective media depending on the bacteria youa re interested in. Some anaerobic/microaerophilic conditions wouldn't hurt either. It all depends on what you are looking for.

LB is probably not the best choice.
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Post by purna » Tue Apr 25, 2006 5:52 am

is nutrient agar good to use?
can i resuspend th pellet in nutrient broth n the plate the bacteria?

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canalon
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Post by canalon » Tue Apr 25, 2006 5:37 pm

You could do that, but it is better to resuspend pellet in a non nutritive solution,so that they do not start to reproduce before you plate them, and since all bacteria are not reproducing at the same rate in a given media, this will bias your population.

As for the nutrient agar, it's the same as LB. You will get bacteria, sure but which? It is going to be hard to say. You should definitely define what kind of bacteria you are interested in, and the choose an appropriately selective medium. Hence the first conclusion is what do you want to do with your sample?
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Post by angel » Tue Apr 25, 2006 5:57 pm

Canalon wrote:You could do that, but it is better to resuspend pellet in a non nutritive solution,so that they do not start to reproduce before you plate them, and since all bacteria are not reproducing at the same rate in a given media, this will bias your population.

As for the nutrient agar, it's the same as LB. You will get bacteria, sure but which? It is going to be hard to say. You should definitely define what kind of bacteria you are interested in, and the choose an appropriately selective medium. Hence the first conclusion is what do you want to do with your sample?

Sir, I have a querry. The bacteria which are used in sewage plants to decompose sewage are mainly anaerobic bacteria, so if a sewage sample is taken to grow bacteria , will they survive in oxygen.

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canalon
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Post by canalon » Tue Apr 25, 2006 6:02 pm

There are plenty of bacteria in sewage samples. Both anaerobic and aerobic. If you are interested in the anaerobic one you indeed better be carefull when handling your sample if you want to have a chance to culture anything.

Again all this depends on what you are interested in. So the first part in experimental design is to ask yourself what you want, and then to design your experiment so as to be sure to get to this result. If you are interested in DNA, anaerobiosis will not be a problem, if you want to culture aerobic bacteria, it will be the same, but if you are interested in anaerobes, better start seeing if you can put your hands on an anerobic chamber...
Patrick

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Post by angel » Tue Apr 25, 2006 6:12 pm

Canalon wrote:There are plenty of bacteria in sewage samples. Both anaerobic and aerobic. If you are interested in the anaerobic one you indeed better be carefull when handling your sample if you want to have a chance to culture anything.

Again all this depends on what you are interested in. So the first part in experimental design is to ask yourself what you want, and then to design your experiment so as to be sure to get to this result. If you are interested in DNA, anaerobiosis will not be a problem, if you want to culture aerobic bacteria, it will be the same, but if you are interested in anaerobes, better start seeing if you can put your hands on an anerobic chamber...

Thanks for the information

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