Toll-like receptor (TLR) -2 blocking experiment

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Marcel
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Toll-like receptor (TLR) -2 blocking experiment

Post by Marcel » Mon Apr 24, 2006 7:52 am

I'm trying to block the cytokine production of human PBMCs stimulated with TLR-2 agonists using the eBioscience TLR-2 antagonist TLR2.1.
Before stimulation with the agonist, I pre-incubate with the TLR2.1 for 30 min at room temperature at a concentration of 10microgram/ml.

Unfortunately, it doesn't work. Does anybody have an idea what I'm doing wrong?

Thanx,

Marcel

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