Insulin questions

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G Campbell
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Insulin questions

Post by G Campbell » Sat Apr 22, 2006 10:36 am

What are the enzymes that are used for cutting Insulin? Am I right in thinking that EcoR1, BamH1, and Hind111 are the ones that cut it and what is the vector that can be used for Insulin?

angel
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Post by angel » Sat Apr 22, 2006 7:28 pm

Insulin is a protein . How can restriction endonucleases cut it?
Are you refering to insulin Gene???

G Campbell
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Post by G Campbell » Sun Apr 23, 2006 11:47 am

yeah i am refering to the gene

d00d
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Post by d00d » Mon Apr 24, 2006 1:49 am

yeah, those are valid restriction enzymes.
the vector is a plasmid (or circular DNA) obtained from a bacterium.
Since when did what we pay for coloured cloth gauge our gravity?

G Campbell
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Post by G Campbell » Fri Apr 28, 2006 8:21 pm

Could someone give me any good sites that explain about inserting a vector into E.coli? I have looked but nothing good enough seems to come up.

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