Unhealthy C2C12

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maddensd
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Unhealthy C2C12

Post by maddensd » Thu Apr 20, 2006 4:28 pm

I am worried about my C2C12 myoblasts at the moment - they were looking "spindley" last week so I got rid of them, made up fresh media and fresh PBS and broke out a fresh batch of cells. However they still looked the same today (please see picture attached). Should I be worried? I am maintaining them in DMEM (Sigma #D6429) supplemented with 20%FBS, 2mM glutamine and Pen/Strep. I use trypsin to lift them. I am just sub-culturing them at the moment and am not trying to differentiate them. I'm also maintaining HeLa cells at the moment and they look fine.

Thanks for you attention.
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Shu
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unhealthy C2C12

Post by Shu » Fri May 26, 2006 8:21 am

i've been working with this cell line for a while and might be able to help you. however, i can't seem to download the images that you've attached to your post.

daniel.kurz
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Post by daniel.kurz » Fri May 26, 2006 3:37 pm

I can't get the images either.

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MrMistery
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Post by MrMistery » Fri May 26, 2006 5:31 pm

Try attaching your pictures in a *.jpg format. Most browsers can't read *.TIF
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

Shu
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Post by Shu » Sat May 27, 2006 12:51 am

you guys are all working on C2C12 as well?

i just got a new line in, NSC-34s. has anyone had experience with these?

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