Need help with a question on pBR322

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northaustin
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Need help with a question on pBR322

Post by northaustin » Thu Apr 20, 2006 2:55 pm

The question is from an old test that I'm reviewing and reads as follows:

Code: Select all

When a cloned gene is inserted into the tetracycline-resistant gene of pBR322, the result is a cell that:

A) produces tetracycline

B) has lost its plasmid

C) is sensitive to tetracycline

D) is resistant to tetracycline



If you know the answer, and more importantly, can explain it to me, I would be very grateful. Thanks in advance.

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canalon
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Post by canalon » Thu Apr 20, 2006 3:20 pm

USing your brain doesn't actually hurt you know...
Now read the question:

"When a cloned gene is inserted into the tetracycline-resistant gene of pBR322"
What do you think happen to the tetR gene? Deduce consequences for the cell.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

northaustin
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No direct sunlight

Post by northaustin » Thu Apr 20, 2006 4:25 pm

I'm fairly sure that there are questions on topics (e.g. Computational Geometry) that would be obvious to me and give you trouble. In that case I don't think the answer "use your brain" would do you much good if you had questions in this area. The point of posting the question to this forum was that I assumed it would be read by people with expertise, but apparently the "expertise" comes with a lack of civility. The great thing about cyberspace is that one has many options for finding civil people willing to share their knowledge . You can take your forum and your bad attitude and stick them somewhere that doesn't get direct sunlight.

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canalon
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Post by canalon » Thu Apr 20, 2006 5:44 pm

First of all, we prefer to use a civil language on this board. But the rule is "we do not give answer' We may help, but we don't give answers.

Second, I am sure that I would suck in computational geometry, but the problem is this question is really basic. Really. Same as "in a plane 2 lines are paralelles, I draw a perpendicular to the first, what is its position relatively to the other".
In fact I added emphasis when citing the question to try to help you figure out the answer. ANd added a few questions for the same reason.
I repeat and add some more:
- What happens to the gene (tetracycline resistance)?
- What would most likely happent to the product?
- What is tet-resistant gene?
- So what are the consequence of the insertion to the cell?
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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