PEG

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HELISA
Death Adder
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Joined: Sat Feb 25, 2006 12:55 am
Location: Illinois

PEG

Post by HELISA » Tue Apr 18, 2006 12:31 am

Hi,
I'm using PEG for introducing RNA molecule into mesophyll protoplasts and I was wondering how it is possible, that actually cells uptake such a large molecules like RNA or DNA from the environment if PEG is used. What changes in membrane composition this polymer cause, that this procedure is possible? Any ideas?
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2810712
King Cobra
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Post by 2810712 » Tue Apr 18, 2006 3:15 pm

I've heard that PEG is used to fuse cells when cell wall has been removed...PEG, does that get inside the lipid bilayer as is has long chains & due to many sp2 hybridised carbons [ double bonds] it will have many kinks to increase membrane fluidity which may account for easier & faster fusion of cell membranes...my explanation...just nascent...but how do we relate the increased fluidity to more -NA [DNA & or RNA ] absorption?

still i doubt its hydrophbicity & ability to get inside the lipidlayers..it may be in between the hydrophobic and philics , such that its able to get inside bilayer & at the same time, create easy diffusable path through the membrane for -NAs it & having some sort of arrangement inside membrane...or even creating some sort of pores..?

Interesting...gonna search for!

hrushikesh

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