RNA Gel Electrophoresis

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
ilse
Garter
Garter
Posts: 1
Joined: Sat Apr 15, 2006 7:32 pm

RNA Gel Electrophoresis

Post by ilse » Sat Apr 15, 2006 7:45 pm

Hello!

I have a question related to RNA Gel Electrophoresis:
After RNA preparation of trichostrongyle nematodes, I load 200 ng of the RNA on an agarose gel.
I use the 0.24–9.5 Kb RNA Ladder from Invitrogen.
I see 4 bands on the gel: a bright one on +/- 2500 bp (28S), another bright one on +/- 1500 bp (18S), a weak one on +/- 150 bp (is this 5S RNA??) and very weak: +/- 4500 bp (what is this???)

When I look for the length of 28S on the internet, I find that 28S is +/- 4500 bp long. Is this only in mammals?

Can anyone help me?
Thanks

Post Reply

Who is online

Users browsing this forum: No registered users and 5 guests