[Seeking advise] About locating a sequence from vector

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[Seeking advise] About locating a sequence from vector

Post by bbs_r » Thu Apr 06, 2006 12:07 pm

Hi there.

Since the result from my sequencing reaction is unsatisfactory, I would like to locate the target sequence via comparing my sequence with an official vector sequence. So that I might get an idea about where the target/inserted sequence could roughly be.

Does it sound like a good idea? or do you have any better ideas if you were me?

Thank you very much!

PS. Since the experimental period of my project is passed, I can't do any more experiments

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Inland Taipan
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Post by canalon » Thu Apr 06, 2006 6:37 pm

The complete sequence of vectors is usually available through the manufacturer website. If you used PCR II from invitrogen (as another post suggests) it is definitely on thei website.

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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Post by dharma » Mon May 22, 2006 11:03 am

i think u can use any vector databases for the specific organisms lide epd , mdb, ddb.

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