molecular biology

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Helix
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molecular biology

Post by Helix » Wed Apr 05, 2006 11:06 am

Hi,
does anyone know what is the maximum length of DNA that can be amplified by PCR? Please post the answer if some one knows.

angel
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Post by angel » Mon Apr 10, 2006 8:56 am

may be its 1000 bp. i am not sure

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canalon
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Post by canalon » Mon Apr 10, 2006 3:34 pm

More than that! I regularly amplify over 2 kb. with good conditions and enzymes, you can go way over that. Probably up to 10 kb.
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Post by Trev » Mon Apr 10, 2006 4:36 pm

it all depends from polymerase and PCR-method. My scientific leader amplified more than 16 kb.
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LilKim
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Post by LilKim » Mon Apr 10, 2006 6:23 pm

i've personally amplifed 8KB fragments. the problem is that regular taq has a high error rate (approx. 1 error/1000bp). Therefore, if fidelity is extremely important use hi-fi taq (hi fidelity taq)... which uses MgSO4 (instead of MgCL). Also.. the extension time has to be increased so that taq has enough time to extend....

Helix
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Post by Helix » Tue Apr 11, 2006 6:56 am

My sincere thanks to all who have responded to my post. Your answers and suggestions will be very much helpful to me.

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