Dominant negative mutation: Tyr is usually mutate to ?

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annabel0830
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Dominant negative mutation: Tyr is usually mutate to ?

Post by annabel0830 » Tue Apr 04, 2006 2:59 pm

Hi, everyone:

I am designing some dominant-negative mutations.
I want to inactive an enzyme by replace its active site Tyr to another Amino acid, but I don't know which one to choose. I found in papers, Tyr was usually changed to Phe, while sometimes it was changed to Ala. I wonder whether both of them are ok?
I also want to mutate Ser site, I got puzzled again.
Is there any rule to make such decision? Thank you!

Annabel

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