DNA extraction

Genetics as it applies to evolution, molecular biology, and medical aspects.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
biology_06er
Coral
Coral
Posts: 260
Joined: Tue Mar 21, 2006 9:33 am

DNA extraction

Post by biology_06er » Mon Apr 03, 2006 2:42 am

Hi

Could someone please help me out with these questions?

1)Once the DNA has been extracted from an organism (i.e. a plant) why is it neccessary to use the polymerase chain reaction (PCR) before you can detect the gene on an electrophoresis gel?

2)Describe the pattern of DNA fragments on an electrophoresis gel that you would expect from plants without the Bt gene (wild type)?

3)Describe the pattern of DNA fragments on an electrophoresis gel that you would expect from plants which are hybird between wild type and MON810 plants?

Thanks alot
biology_06er

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Mon Apr 03, 2006 1:55 pm

1- What does PCR do? Think of the role of primers.

2- No answer if you do not know which primers are used. But basically, see which genes could be amplified with our primers, and which will not (of course gene that are absent are not amplified)

3- Do you punnet square to figure out the genotype of your pant in respect to the genes amplified by your primers, and you wil get the pattern.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

biology_06er
Coral
Coral
Posts: 260
Joined: Tue Mar 21, 2006 9:33 am

Post by biology_06er » Wed Apr 05, 2006 7:19 am

Thanks for comment

Post Reply

Who is online

Users browsing this forum: No registered users and 5 guests