Guanidine hydrochloride

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HELISA
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Guanidine hydrochloride

Post by HELISA » Sat Apr 01, 2006 1:23 am

Another question concerning DNA extraction.
Guanidine hydrochloride is used in the presence of ethanol to precipitate DNA, why this reagent (which is so harmful) is used instead of i.e. NH4Ac?

angel
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Post by angel » Sat Apr 01, 2006 4:22 am

Guanidium hydrochloride is more useful in RNA extraction. As DNA precipitation in presence of ethanol is concerned, sodium acetate is a much better choice. Ammonium acetate may cause some problems during PCR reaction.

HELISA
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Post by HELISA » Sun Apr 02, 2006 12:00 am

So, why the guanidine hydrochloride is the ingredient of AP3/E buffer used in DNeasy kit?
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Post by angel » Mon Apr 03, 2006 9:38 am

if you are using kits then i may not help. i was refering to general laboratory practice in which the salt is expensive as well as non essential.

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