RNA degradation with alkali?

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bbs_r
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RNA degradation with alkali?

Post by bbs_r » Thu Mar 30, 2006 7:55 am

According to:
Image


How does the akali destroys mRNA in the hybrid? Does it means "the bonding of nucleotides in mRNA degrades naturally in a high pH"?

Or I had misunderstood its meaning?

Thank you very much!

Xgene
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Post by Xgene » Thu Mar 30, 2006 8:31 pm

mRNA has ribose sugar in its nucleotides, which is susceptable for alkali degradation. where as in the DNA deoxy ribose is present in its nucleotides which will resist alkali degradation.

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