Mammalian mutagenesis?

Genetics as it applies to evolution, molecular biology, and medical aspects.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
User avatar
LilKim
Coral
Coral
Posts: 436
Joined: Mon Mar 20, 2006 8:36 pm
Contact:

Mammalian mutagenesis?

Post by LilKim » Tue Mar 28, 2006 4:37 pm

Does anyone know if any literature that have effectively isolated a gene in MAMMALIAN cells (via. mutagensis and genetic screening)?

(I know how to do it in drosph, yeast, c. elegans etc etc etc ... )

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Tue Mar 28, 2006 11:16 pm

What do you mean isolated a gene in mammalian cell?

Because if:
- it is taking a gene from a mammal and cloning it, it has been done (recombinant insulin for example).

- it is inserting a gene in a mmal cell, it has also been done (gfp pigs for example, but also goats producing drugs in their milk etc)

otherwise, be a little more precise, but the answer will probably be yes, and you could probably find it on pubmed (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi)
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

User avatar
LilKim
Coral
Coral
Posts: 436
Joined: Mon Mar 20, 2006 8:36 pm
Contact:

Post by LilKim » Thu Mar 30, 2006 2:01 am

oK... lemme re-word my question.

I need to set up a genetic screen using mammalian cells. Essentially, I want to use a genetic approach to determine if the RITS (RNAi induced transcriptional silencing) complex exists in mammalian cells (last year it was Identified in yeast and published in Sciencemag)

I did a quick pubmed search looking for any genetic screens performed on mammalian cells. And I essentially came up with nothing (...from respectable journals)

I talked to my professor, and he says it's do-able... i'll just have to figure out how the hell to do it??? (this is actually for an assignment.. i have to write a proposal)

... it's seems like it would be really difficult technically... (i'm already seeing sleepless nights... trying to design this) So, I was wondering if anyone had more of a clue than I do... and if they could provide a clue for me to start from.

thanks in advance:
- KIM

Cloak & Dagger
Garter
Garter
Posts: 5
Joined: Mon Apr 10, 2006 2:43 pm

Post by Cloak & Dagger » Mon Apr 10, 2006 4:25 pm

You could look up the DNA sequence of the yeast components of the complex and search for homologues in a mammalian genome.

Then you could target whatever you find for mutagenesis and screen for RNAi induced silencing. That should confirm whether they have the same functions in mammals (at least in the type of cells you are using).

Hope that helps.

User avatar
LilKim
Coral
Coral
Posts: 436
Joined: Mon Mar 20, 2006 8:36 pm
Contact:

Post by LilKim » Mon Apr 10, 2006 6:17 pm

Hey.. thanks for the reply

.... my paper is due in another 2 weeks... and I actually thought of really similar idea.

yeah, i've actually gone and looked for homologs and orthologs and they haven't been identified in mammals. BUT, based on the stuff i've read .. i think it's VERY plausible for the RITS activity in mammalian cells...

I've devised a plan (along the same-lines as what you've said) to solve the problem, and I think it will work. (i'm going to make an appointment to speak with my professor about it..on thursday or friday)

... but, thanks! It's a good feeling to know that 2 different people came to similar conclusions independantly... so I feel encouraged

thanks again & best wishes!
- KIM

Post Reply

Who is online

Users browsing this forum: No registered users and 4 guests