Gene Sequence

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Ajay
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Gene Sequence

Post by Ajay » Sat Mar 25, 2006 6:18 pm

By just looking at a dna sequence can we deduce the gene sequence i.e. coding sequences present in between non coding sequences? If so what are the methods to find by just SEEING it?

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Sepals
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Post by Sepals » Mon Mar 27, 2006 10:24 am

Yeah you can look for the promotors sequences. One is the TATA box which is mostly made of of Ts and As but the no very in differnet organisms and is at -10 bp. The consensus sequence of it (average) being TATAAT. There is another sequence at -35 bp which has the consensus sequence of TTGACA.
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GreenDog
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Post by GreenDog » Mon Mar 27, 2006 11:44 am

We can't say for sure, but we can speculate based on concensus sequences. TATA box is a concensus for a promotor like Sepals said, other sequences mark splice sites etc. Tha way to do it is to use a computer program which takes in account as many parameters as it can to predict the answer to your question.
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Sepals
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Post by Sepals » Tue Mar 28, 2006 12:28 am

Like Bioedit which can be d/l free here:

http://www.mbio.ncsu.edu/BioEdit/bioedit.html
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