DNA fingerprinting

Genetics as it applies to evolution, molecular biology, and medical aspects.

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angel
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DNA fingerprinting

Post by angel » Wed Mar 22, 2006 11:24 am

why we need to take many primers when analysing DNA fingerprint if we can get good polymorphism from one primer only in RAPD

HELISA
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Post by HELISA » Wed Mar 22, 2006 5:50 pm

RAPD method has a lot of disadvantages.
The result of this reaction depends on many factors: the type of PCR machine you use, the company from which you order primers (even if they have the same sequence of primers, but they are from different company, they could give you different products!), the annealing temperature, concentration of Mg ions…simply, everything. As far as I’m concerned, this reaction is very unreliable and the results of the same reactions could be sometimes unrepeatable. So, RAPD is not good for every kind of fingerprinting.

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