SSR or Microsatellite Markers

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Master DRe
Posts: 4
Joined: Wed Mar 15, 2006 9:12 pm
Location: Quebec City, Canada

SSR or Microsatellite Markers

Post by Master DRe » Wed Mar 15, 2006 9:18 pm

Hi everyone,
I'm presently developing SSR markers on conifer species and I'm not able to remove the stutter bands. I have tried to increase the temp, decrease the primer concentration, modified the [MgCl2] etc...
Do you have an advice for me??

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Death Adder
Posts: 67
Joined: Sat Feb 25, 2006 12:55 am
Location: Illinois

Post by HELISA » Mon Mar 20, 2006 1:52 pm

Change the polymerase. Sometimes, enzymes have some kind of contaminations of bacterial DNA which can result in additional bands on your gel. All the best.

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