molecular cloning: blunt ends

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maria_geo
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molecular cloning: blunt ends

Post by maria_geo » Sun Mar 12, 2006 4:08 pm

im studying for a test i have tom on "recombinant DNA technology.Principles of molecular cloning" and i'm trying to find out what the "blunt ends" are. im looking in my dictionaries but i cant find a definition for it.. can someone help me pls? :?

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GreenDog
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Post by GreenDog » Sun Mar 12, 2006 7:19 pm

Blunt ends in DNA are created when there no overhang is left. (Usually as a result of a restriction enzyme function) For example:
Blunt End:
CCG
GGC
Sticky end:
CCGCGG
GGC
"When In Danger Or In Doubt Run In Circles Scream And Shout"
Lawrence J. Peter

maria_geo
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Post by maria_geo » Sun Mar 12, 2006 7:37 pm

thnx a lot green dog..:)

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mith
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Post by mith » Tue Mar 14, 2006 12:27 am

ones with overhang are sticky ends :)
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